FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_16.3510000005cb23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_16.3510000005cb23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC201.6No Hit
GAACACCATAGCGCCTGTCTCTTATAC80.64No Hit
GAACACCATAGCGGCTGTCTCTTATAC60.48No Hit
GAACACCATAGCAGCTGTCTCTTATAC60.48No Hit
GAACACCATAGCGTCTGTCTCTTATAC50.4No Hit
GAACACCATAGCAACTGTCTCTTATAC50.4No Hit
GAACACCATAGCACCTGTCTCTTATAC40.32No Hit
GAACACCATAGCCCTGTCTCTTATACA40.32No Hit
GAACACCATAGCTCCTGTCTCTTATAC40.32No Hit
GAACACCATAGCTGTCTCTTATACACA40.32No Hit
GAACACCATAGCCTCTGTCTCTTATAC40.32No Hit
GAACACCATAGCCCCTGTCTCTTATAC40.32No Hit
GAACACCATAGCTACTGTCTCTTATAC30.24No Hit
GAACACCATAGCCAGGGGGGTTTTTTT30.24No Hit
GAACACCATAGCGGGGATGGTTTTTTT20.16No Hit
GACCACCATAGCCCCTGTCTCTTATAC20.16No Hit
GAACACCATAGCGGGGGGTATTTTTTT20.16No Hit
GAACACCATAGCGGTAGGGGTTTTTTT20.16No Hit
GAACACCATAGCGTGTGGGGCTGTCTC20.16No Hit
GAACACCATAGCTTCTGTCTCTTATAC20.16No Hit
GAACACCATAGCGCAGTGGGTTTTTTT20.16No Hit
GAACACCATAGCCGCTGTCTCTTATAC20.16No Hit
GAACACCATAGCATCTGTCTCTTATAC20.16No Hit
GAACACCATAGAGTCACGTACCTGTCT20.16No Hit
GAACACCATAGCGTGGTCGATTTTTTT20.16No Hit
GAACACCATAGCGGGTGGGATCTGTCT20.16No Hit
GAACACCATAGCGGGGGCGATTTTTTT20.16No Hit
GAACACCATAGCTGTGGGAATTTTTTT20.16No Hit
GAACACCATAGCAGTGCGGGTTTTTTT20.16No Hit
GAACACCATAGCGGGGTGTGTTTTTTT20.16No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTTT301.6740938E-421.00000219
CACCATA1200.021.0000024
ACCATAG1250.021.05
TTTTTTT850.021.021
GTTTTTT401.1890661E-621.020
ACACCAT1050.021.03
ATAGCGG401.1890661E-621.08
GAACACC1050.021.01
CATAGCG750.021.07
CCATAGC1250.021.06
AACACCA1050.021.02
ATTTTTT250.001853412620.99999820
GCTGTCT300.005364295617.50000220
Produced by FastQC (version 0.11.2)