FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_15.3510000005cb09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_15.3510000005cb09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12305
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA1190.9670865501828526No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT320.26005688744412836No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT230.1869158878504673No Hit
TGGCATAGCTGAGGGCCTGTCTCTTAT220.17878911011783827No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC180.1462819991873222No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT150.12190166598943519No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT140.11377488825680616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCATA10600.021.2975221
GGCATAG10600.021.2975222
GCCTAGC1050.021.2975223
GGCCTAG1050.021.2975222
TGGCCTA1050.021.2975221
GCATAGC10700.021.2975223
GCTGAGC1450.020.9512218
TGGTTTT303.6948884E-420.9512218
GTGTTTT250.003239257720.9512218
CTGAGTG455.5198325E-720.951229
CTGAGTA303.6948884E-420.951229
CTGAGGT557.206836E-920.951229
CTGAGGC250.003239257720.951229
CTGAGGA506.310256E-820.951229
CTGAGCT250.003239257720.951229
CTGAGCG557.206836E-920.951229
CTGAGCA250.003239257720.951229
CTGAGAG557.206836E-920.951229
AATTTTT506.310256E-820.9512219
TGTTTTT1000.020.9512219
Produced by FastQC (version 0.11.2)