FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_14.3510000005caee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_14.3510000005caee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12433
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTCTGTTGCTGTCTCTTATACACA1301.0456044397973137No Hit
ATGCTCTGTTGCGGGGCTGTCTCTTAT250.20107777688409878No Hit
ATGCTCTGTTGCGGCTGTCTCTTATAC240.19303466580873482No Hit
ATGCTCTGTTGCGGGCCTGTCTCTTAT160.12868977720582322No Hit
ATGCTCTGTTGCCTGTCTCTTATACAC150.12064666613045927No Hit
ATGCTCTGTTGCGGGGGGGGTTTTTTT150.12064666613045927No Hit
ATGCTCTGTTGCAGCTGTCTCTTATAC150.12064666613045927No Hit
ATGCTCTGTTGCTGTCTCTTCTACCCA140.11260355505509533No Hit
ATGCTCTGTTGCGCCTGTCTCTTATAC130.10456044397973137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTGT11750.021.220413
ATGCTCT11250.021.2204071
TGCTCTG11300.021.1265132
TGTTGCG5900.020.963717
TGTTGCA2250.020.963717
TGCGCGG303.6824093E-420.9637110
TGTTTTT1150.020.9637119
TCTGTTG12150.020.963715
GCTGTTT303.6824093E-420.9637117
TCGTTTT303.6824093E-420.9637118
GCGTTTT303.6824093E-420.9637118
AGGTTTT303.6824093E-420.9637118
TTGCGTG303.6824093E-420.963719
TTGCGTC303.6824093E-420.963719
GGGGTGT303.6824093E-420.9637115
TTGCGAC303.6824093E-420.963719
GTTGCTA303.6824093E-420.963718
GTTGCCT303.6824093E-420.963718
GTTGCCC303.6824093E-420.963718
GTTGCAG1200.020.963718
Produced by FastQC (version 0.11.2)