FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_13.3510000005cac4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_13.3510000005cac4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10987
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTCTGTCTCTTATACACA700.6371165923363975No Hit
GACTCTATGGTGGCTGTCTCTTATACA510.4641849458450896No Hit
GACTCTATGGTGCCTGTCTCTTATACA480.4368799490306726No Hit
GACTCTATGGTGACTGTCTCTTATACA250.227541640120142No Hit
GACTCTATGGTGTCTGTCTCTTATACA190.1729316464913079No Hit
GACTCTATGGTGCTGTCTCTTATACAC120.10921998725766816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAT9450.021.16592
CTCTATG9500.021.16593
GATTCTA353.8465183E-521.16591
TTCTATG353.8465183E-521.16593
ATTCTAT353.8465183E-521.16592
GACTCTA9450.021.16591
CTATATG657.8216544E-1121.1658973
GACTATA657.8216544E-1121.1658971
ACTATAT657.8216544E-1121.1658972
GGTGACG250.003197563820.9726059
AATTTTT506.081791E-820.97260519
TGATTTT250.003197563820.97260518
TTCTTTT250.003197563820.97260518
TGCTTTT250.003197563820.97260518
GCGTTTT250.003197563820.97260518
GGTGGTA250.003197563820.9726059
TGGTGTC250.003197563820.9726058
TGGTGCT506.081791E-820.9726058
GTGTGGG250.003197563820.97260510
GGTGCGA250.003197563820.9726059
Produced by FastQC (version 0.11.2)