FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_12.3510000005caab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_12.3510000005caab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15394
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC1120.7275561907236586No Hit
GAATCCTTACGCCTGTCTCTTCTACAC230.14940886059503702No Hit
GAATCCTTACGCTGTCTCTTATACACA210.13641678576068597No Hit
GAATCCTTACGCCCTGTCTCTTATACA200.12992074834351047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCCT13050.021.1776331
AATCCTT13000.021.1776312
CTCCTTA607.6033757E-1021.1776313
ACTCCTT607.6033757E-1021.1776312
GTATCCT556.681148E-921.1776311
TTCCTTA353.965092E-521.1776313
TATCCTT556.681148E-921.1776312
GATTCCT353.965092E-521.1776311
GAATACT607.6033757E-1021.1776311
ATACTTA607.6033757E-1021.1776313
ATTCCTT353.965092E-521.1776312
GACTCCT607.6033757E-1021.1776311
ATCCTTA13550.021.1776313
ACGCTTA250.003258502820.9706869
ACGCCGA250.003258502820.9706869
ACGCTGG651.0004442E-1020.9706869
ACGCTGC250.003258502820.9706869
ACGCTAA250.003258502820.9706869
TTTCTGT651.0004442E-1020.97068621
GTTCTGT250.003258502820.97068620
Produced by FastQC (version 0.11.2)