FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_11.3510000005ca81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_11.3510000005ca81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13945
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGTGGCTGTCTCTTATAC200.14342058085335246No Hit
AAGTGCCTCAGCTGTCTCTTATACACA180.1290785227680172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCTC13000.021.3897083
ATGTGCC303.2367892E-421.3897061
AAGTGCC12700.021.3897061
TGTGCCT303.2367892E-421.3897062
AGTGCCT12750.021.3058242
GTTTTTT3350.021.0036120
ATCTGTC250.00321308421.0036120
CTTTTTT2000.021.0036120
GCCTCAG13450.020.9280595
CAGTTAT404.950696E-620.9280599
AGTGGGG455.6904173E-720.92805910
GGTTTTT900.020.92805919
ACTTTTT404.950696E-620.92805919
TCAGTTC455.6904173E-720.9280598
TCAGTTA800.020.9280598
TCAGTGT1450.020.9280598
TCAGTGG1950.020.9280598
TCAGTGC900.020.9280598
TGCCTCA13450.020.9280594
TCAGTCA404.950696E-620.9280598
Produced by FastQC (version 0.11.2)