FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate3_10.3510000005ca67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate3_10.3510000005ca67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6840
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC650.9502923976608186No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC300.43859649122807015No Hit
ACGTCTCTGCCTGTCTCTTATACACAT290.42397660818713445No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT70.10233918128654972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTC5900.021.2686561
GTATCTG403.7712434E-621.2686563
ACGTATC403.7712434E-621.2686561
CGTATCT403.7712434E-621.2686562
CGTCTCT5900.021.2686562
GTCTCTG6100.021.0943243
CTCTGCA6150.020.9558835
TGCATTT353.8623213E-520.9558838
TGCATTC303.4566142E-420.9558838
GGGTTTT606.4028427E-1020.95588318
TGCATGC656.91216E-1120.9558838
TGCATAG656.91216E-1120.9558838
TTTTTTT4900.020.95588321
TGTTTTT606.4028427E-1020.95588319
TCTGCAT6600.020.9558836
GGCTTTT303.4566142E-420.95588318
GCATGGC303.4566142E-420.9558839
GATTTTT303.4566142E-420.95588319
GCATGCA303.4566142E-420.9558839
GCATGAG353.8623213E-520.9558839
Produced by FastQC (version 0.11.2)