FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_96.3510000005c925.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_96.3510000005c925.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11316
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC1571.3874160480735243No Hit
TAAGCGTGGAACCTGTCTCTTCTACAC700.6185931424531637No Hit
TAAGCGTGGAACTGTCTCTTATACACA200.17674089784376104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT9950.021.416291
TAAGAGT707.2759576E-1221.416291
AAGCGTG10000.021.319822
AGCGTGG10050.021.319823
AAGAGTG707.2759576E-1221.3198182
AGAGTGG707.2759576E-1221.3198183
TTTCTGT303.6791863E-420.94247821
GAACGTG303.6791863E-420.9424789
GAACGTC303.6791863E-420.9424789
TTCTGTC303.6791863E-420.94247821
GAACGGG608.039933E-1020.9424789
GAACGGA303.6791863E-420.9424789
AGTGGAA701.0913936E-1120.9424785
GAACGCA354.194941E-520.9424789
GAACGAC303.6791863E-420.9424789
GGTTTTT1100.020.94247819
GAGTTTT354.194941E-520.94247818
GCGTGGA10550.020.9424784
CGTGGAA10550.020.9424785
GAACCTG354.194941E-520.9424789
Produced by FastQC (version 0.11.2)