FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_94.3510000005c8e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_94.3510000005c8e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123960
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA3460.2791222975153275No Hit
TACTGCAAGGAGGCTGTCTCTTATACA1290.10406582768635045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAAG2000.021.53
ACAGCAA250.002933569321.52
ACTGCAA113200.021.52
CAGCAAG250.002933569321.53
TACAGCA250.002933569321.51
TTCTGCA950.021.51
CTCCAAG950.021.53
ACTCCAA950.021.52
TCTGCAA950.021.52
TACTGCA113300.021.51
ATGCAAG1250.021.53
AATGCAA1250.021.52
CTGCAAG114100.021.53
TACTCCA950.021.51
TACTACA2000.021.51
ACTACAA2000.021.52
ATTGCAA1800.021.4999982
GGAGATC850.020.918929
AGCCTGG354.6982088E-520.9189211
GAGGTGT850.020.9189210
Produced by FastQC (version 0.11.2)