FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_91.3510000005c883.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_91.3510000005c883.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39082
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA4331.1079269228800983No Hit
CTAGCTGGATGTCTGTCTCTTATACAC1100.2814594954198864No Hit
CTAGCTGGATCTGTCTCTTATACACAT730.18678675605137915No Hit
CTAGCTGGATGTGGGGCTGTCTCTTAT570.14584719308121386No Hit
CTAGCTGGATGTGGGCTGTCTCTTATA540.13817102502430786No Hit
CTAGCTGGATGTGGGGGCTGTCTCTTA540.13817102502430786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA1400.021.162793
CCAGCTG354.199305E-521.162791
GAGCTGG608.8766683E-1021.162792
TCGCTGG1400.021.162792
CTAGCTG34400.021.162791
CGAGCTG608.8766683E-1021.162791
AGATGGA950.021.162793
TAGATGG950.021.162792
CAGCTGG354.199305E-521.162792
TGCTGGA455.629772E-721.1627883
CAAGCTG250.003176426321.1627881
AGCTGGA35700.021.1331523
TAGCTGG34550.021.1321642
TGTCCGG354.5024513E-520.97311210
GAAGTTT354.5024513E-520.97311217
TTTTCTG354.5024513E-520.97311221
ATGGCTG354.5024513E-520.97311217
GATGTGT2850.020.9731128
GATGTGG7500.020.9731128
GATGTGA2750.020.9731128
Produced by FastQC (version 0.11.2)