FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_9.3510000005be30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_9.3510000005be30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71586
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGTACGTAGGCTGTCTCTTATACA4390.6132484005252424No Hit
TACGGTACGTAGCCTGTCTCTTATACA2160.3017349761126477No Hit
TACGGTACGTAGACTGTCTCTTATACA1490.20814125667029865No Hit
TACGGTACGTAGTCTGTCTCTTATACA1490.20814125667029865No Hit
TACGGTACGTAGCTGTCTCTTATACAC1400.19556896599893836No Hit
TACGGTACGTAGGGGCTGTCTCTTATA940.13131059145643004No Hit
TACGGTACGTAGGGCTGTCTCTTATAC930.12991367027072334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG1200.021.4645183
ACGCTAC505.7996658E-821.4645162
TGGTACG1350.021.4645163
TACGGTA65200.021.4645161
TCGGTAC950.021.4645162
ATGGTAC1350.021.4645162
CGCTACG505.7996658E-821.4645163
TACGCTA505.7996658E-821.4645161
ACGGTAC65300.021.4645162
TTCGGTA950.021.4645161
CGGTACG66250.021.4645163
TATGGTA1350.021.4645161
TGGTTTT1200.020.9245318
TAGAACC303.979711E-420.9245310
TAGAACA303.979711E-420.9245310
TAGCGGT303.979711E-420.9245310
TAGCGGC354.652982E-520.9245310
TAGCGCG303.979711E-420.9245310
TTCATTT354.652982E-520.9245317
AGGGGGA354.652982E-520.9245311
Produced by FastQC (version 0.11.2)