FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_87.3510000005c7fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_87.3510000005c7fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39018
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA620.15890102004203188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT850.021.2337681
GTCGTCA850.021.2337683
CGTCGTC850.021.2337682
TTCGTCA33500.021.2337673
ACATCGT608.5128704E-1021.2337671
ACTTCGT32950.021.2337671
ACTTAGT2850.021.2337671
ACCTCGT250.003115043521.2337671
CTTCGTC32950.021.2337672
ATCGTCA608.5128704E-1021.2337673
ATTTCGT557.3250703E-921.2337671
TTAGTCA2850.021.2337673
CTCGTCA250.003115043521.2337673
TTTCGTC557.3250703E-921.2337672
CATCGTC608.5128704E-1021.2337672
CAGCTCC1700.020.961548
AGCTTGT850.020.961549
AGCTTGC850.020.961549
AATTTTT1650.020.9615419
TAGTCAG2850.020.961544
Produced by FastQC (version 0.11.2)