FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_86.3510000005c7d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_86.3510000005c7d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21450
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA870.4055944055944056No Hit
TGTTGCCGAACACCTGTCTCTTATACA300.13986013986013987No Hit
TGTTGCCGAACAGCTGTCTCTTATACA230.10722610722610723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCC19100.021.5625021
TTGCCGA19200.021.5625023
TTACCGA353.531402E-521.56253
TGTTACC353.531402E-521.56251
TGGTGCC709.094947E-1221.56251
GTTACCG353.531402E-521.56252
GTTGCCG19050.021.56252
GGTGCCG709.094947E-1221.56252
GTGCCGA709.094947E-1221.56253
ACAGCGG405.206506E-620.90909210
TGGTTTT701.2732926E-1120.90909218
GTGTTTT950.020.90909218
AACAACG354.4859247E-520.9090929
ACATGGA303.8748054E-420.90909210
AATTTTT609.567884E-1020.90909219
TTTCTGT558.212737E-920.90909221
TGCCGAA20850.020.9090924
GTTCTGT354.4859247E-520.90909220
GGTCTGT456.0540697E-720.90909219
GCGGTTT405.206506E-620.90909217
Produced by FastQC (version 0.11.2)