FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_85.3510000005c7ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_85.3510000005c7ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33359
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA1100.32974609550646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA29650.021.3297712
CTCTCGT1600.021.3297711
CTCTTGT250.003024982521.3297711
CTCTAGT29650.021.3297711
TCTCGTA1600.021.3297712
ATAGTAC303.4404025E-421.3297713
TCTTGTA250.003024982521.3297712
CTTGTAC250.003024982521.3297713
CCTAGTA353.928398E-521.3297712
TATAGTA303.4404025E-421.3297712
CTCGTAC1600.021.3297713
CCCTAGT353.928398E-521.3297711
CTAGTAC30200.021.329773
TGGTTTT900.020.94602818
TCCTTTT250.003362156220.94602818
CAGTTTT701.4551915E-1120.94602818
TAAATTT250.003362156220.94602817
CGATAGC250.003362156220.94602810
TTACTTT250.003362156220.94602817
CCAGTTT303.8913672E-420.94602817
Produced by FastQC (version 0.11.2)