FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_84.3510000005c791.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_84.3510000005c791.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62046
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA2790.449666376559327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACG2500.021.2055471
GAACGAC54800.021.2055473
AGAACGA54450.021.2055472
GACCGAC2650.021.2055473
CAGACCG2650.021.2055471
AGACCGA2650.021.2055472
CAGAACG54300.021.2055471
GAGCGAC250.003160270921.2055453
TAACGAC850.021.2055453
ATAACGA850.021.2055452
CAGAGCG250.003160270921.2055451
CTGAACG404.847274E-621.2055451
AGAGCGA250.003160270921.2055452
TGAACGA404.847274E-621.2055452
CATAACG850.021.2055451
ACAGTGT5600.020.9661318
ACAGTGG11250.020.9661318
AGCTTTT701.4551915E-1120.96613118
CAGTCAG701.4551915E-1120.9661319
CAGTTAG701.4551915E-1120.9661319
Produced by FastQC (version 0.11.2)