FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_83.3510000005c777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_83.3510000005c777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63228
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA4930.779717846523692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGG557.697963E-921.19843
CACACAA404.863974E-621.19841
ACAGAAG56150.021.19842
CACAAGG404.863974E-621.19843
CACCGAA4000.021.19841
ACCGAAG4000.021.19842
ACACAAG404.863974E-621.19842
TCAGAAG1000.021.19842
CTCAGAA1000.021.19841
ATAGAAG557.697963E-921.19842
AAGAAGG250.003167142221.19843
CAAAGAA250.003167142221.19841
CATAGAA557.697963E-921.19841
CAGAAGG57050.021.19843
CACAGAA56050.021.19841
AAAGAAG250.003167142221.19842
CCGAAGG4000.021.19843
AATTCTG303.9019206E-420.98020719
ACGTTTT601.02591E-920.98020718
CTCTTTT303.9019206E-420.98020718
Produced by FastQC (version 0.11.2)