FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_82.3510000005c75e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_82.3510000005c75e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21781
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC900.4132041687709471No Hit
ACTCGTCTCGATCTGTCTCTTCTACAC400.18364629723153209No Hit
ACTCGTCTCGATGCTGTCTCTTATACA230.10559662090813093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC303.3781677E-421.3372381
CCGTCTC303.3781677E-421.3372383
CCCGTCT303.3781677E-421.3372382
ACTCGTC19450.021.3372361
TCGTCTC19650.021.3372363
GCGTCTC658.54925E-1121.3372363
ACGCGTC658.54925E-1121.3372361
CTCGTCT19450.021.3372362
CGCGTCT658.54925E-1121.3372362
TCGATCG1900.020.944838
CGATGGT950.020.944839
GATGGGT303.8327722E-420.94482810
GATGGGG558.069037E-920.94482810
GATGGGC303.8327722E-420.94482810
GATGGAG303.8327722E-420.94482810
GATGGAC250.003326089620.94482810
GCCTTTT250.003326089620.94482818
CAGTTTT405.135562E-620.94482818
CGATATG303.8327722E-420.9448289
AGCTTTT250.003326089620.94482818
Produced by FastQC (version 0.11.2)