FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_81.3510000005c727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_81.3510000005c727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45268
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA1210.26729698683396663No Hit
AACGGAATGGCTGCTGTCTCTTATACA820.18114341256516744No Hit
AACGGAATGGCTCCTGTCTCTTATACA750.16567995051692144No Hit
AACGGAATGGCTGTCTCTTATACACAT530.11708049836529116No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAT353.7738257E-521.4693892
ATCGGAA353.7738257E-521.4693891
AACGAAA505.6646968E-821.4693871
ATGGAAT556.51562E-921.4693872
CGGAATG42500.021.4693873
AAAGGAA250.002928561521.4693871
AATGGAA556.51562E-921.4693871
AACGGAA42150.021.4693871
ACGGAAT42150.021.4693872
TTGTTTT701.4551915E-1120.92375818
CAGTTTT303.9495164E-420.92375818
ATCTTTT354.6057845E-520.92375818
CTGAGGG405.390797E-620.92375811
TATATTT303.9495164E-420.92375817
ACGTTTT354.6057845E-520.92375818
AGGATTT303.9495164E-420.92375817
GCTAGAG303.9495164E-420.92375810
CTGGGGT354.6057845E-520.92375811
CTGGGGA354.6057845E-520.92375811
GCTGGTC405.390797E-620.92375810
Produced by FastQC (version 0.11.2)