FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_80.3510000005c71a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_80.3510000005c71a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41321
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC1310.3170300815565935No Hit
GGATAGTTAGCCTGTCTCTTATACACA620.15004477142373127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTTA1500.021.4683253
GCTAGTT2050.021.4683232
GTATAGT353.7646823E-521.4683231
ATAGTTA35100.021.4683233
GGCTAGT2050.021.4683231
ATCGTTA1450.021.4683233
GGATAGT34900.021.3760531
GATAGTT34950.021.3454722
CTAGTTA2100.020.9571723
TTTCTGT601.0149961E-920.9478821
AGCCCGT651.2187229E-1020.922529
AGCCCCG651.2187229E-1020.922529
AGCCCAC405.3746644E-620.922529
AGCCTAT800.020.922529
TCCTTTT250.003397693420.9225218
AGCCTAA405.3746644E-620.922529
GGCATTT250.003397693420.9225217
GCCTTAG250.003397693420.9225210
AGCCATG507.412564E-820.922529
AGCCATC405.3746644E-620.922529
Produced by FastQC (version 0.11.2)