FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_8.3510000005be16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_8.3510000005be16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50531
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC9071.7949377609784094No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC1890.3740278245037699No Hit
ACAGTGCTCATGGCTGTCTCTTATACA940.18602442065266866No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC910.18008747105737072No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC550.1088440759137955No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC520.10290712631849756No Hit
ACAGTGCTCCTGTCTCTTATACACATC520.10290712631849756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCTC303.417867E-421.3822063
CAGTGCT44250.021.3822062
ACAGCGC353.9065286E-521.3822041
ACTGTGC701.0913936E-1121.3822041
CCGTGCT3300.021.3822042
ACCGTGC3300.021.3822041
AGTGCTC44550.021.3822043
ATAGTGC404.48183E-621.3822041
AGCGCTC353.9065286E-521.3822043
CAGCGCT353.9065286E-521.3822042
ACAGTGC44350.021.3822021
CGTGCTC3350.021.0630663
ATGGGTC601.036824E-920.93762610
TGCATTT303.9406505E-420.93762617
ATGGGAA303.9406505E-420.93762610
CATGAGC601.036824E-920.9376269
CATGAGA601.036824E-920.9376269
CATGACA303.9406505E-420.9376269
AGCTCAT303.9406505E-420.9376265
GGTCTTT303.9406505E-420.93762617
Produced by FastQC (version 0.11.2)