FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_79.3510000005c6ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_79.3510000005c6ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45664
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAAAAAAAAAAAAAAAAA1060.23213034337771551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCAT1500.021.1390741
AGCCATC1500.021.1390742
GAAATCG1950.021.1390743
TGACATC651.07320375E-1021.1390742
CTGACAT651.07320375E-1021.1390741
TACATCG651.07320375E-1021.1390743
AGAAATC1950.021.1390742
GCCATCG1500.021.1390743
CAGAAAT1950.021.1390741
ATACATC651.07320375E-1021.1390742
AGACATC40350.021.1390742
CATACAT651.07320375E-1021.1390741
GACATCG40950.021.1390723
CAGACAT40450.021.1390721
GCAAGTG1300.020.9770019
GCAAGTA751.8189894E-1220.9770019
GCAAGGA1500.020.9770019
GCAAGCC751.8189894E-1220.9770019
CCGTTTT751.8189894E-1220.97700118
TTCTTTT651.1823431E-1020.97700118
Produced by FastQC (version 0.11.2)