FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_78.3510000005c6d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_78.3510000005c6d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45991
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC1610.350068491661412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGA606.9303496E-1021.6096741
AGTGCGA353.589723E-521.6096741
AGTGTGA40750.021.6096741
AGTGAGA303.1749404E-421.6096741
AGGGTGA1100.021.6096731
AGCGTGA658.0035534E-1121.6096731
TGCGACG353.621114E-521.5853943
GTGCGAC353.621114E-521.5853942
TGAGACG303.1992389E-421.5853923
TGTGACG41150.021.5853923
GAGTGAC607.021299E-1021.5853922
GCGTGAC658.0035534E-1121.5853922
GTGAGAC303.1992389E-421.5853922
GTGTGAC40900.021.5853922
AACCGGG900.020.90424510
GTTCTGT456.3900916E-720.90424520
TGTTTTT4150.020.90424519
TAGTTTT1050.020.90424518
GAGTTTT1050.020.90424518
GCAGTTT456.3900916E-720.90424517
Produced by FastQC (version 0.11.2)