FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_77.3510000005c6bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_77.3510000005c6bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54875
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTCCAAGCCTGTCTCTTATACACA4560.8309794988610478No Hit
AAGGTCCAAGCACTGTCTCTTATACAC4310.7854214123006834No Hit
AAGGTCCAAGCAGTGGTATCAACGCAG1040.1895216400911162No Hit
AAGGTCCAAGCAGTGGTATCCTGTCTC640.11662870159453304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCA556.4646883E-921.5060942
GGACCAA556.4646883E-921.5060943
AAGGACC556.4646883E-921.5060941
GGTCCAA51400.021.5060923
AAGGTCC50800.021.5060921
AGGTCCA50800.021.5060922
TGTCCAA353.7434245E-521.506093
ATGGTCC607.4578566E-1021.506091
GGCCCAA800.021.506093
AGGCCCA800.021.506092
AAGGCCC800.021.506091
ATGTCCA353.7434245E-521.506092
TGGTCTG250.003416117320.9179618
TCCTTTT507.543167E-820.9179618
TTCATTT250.003416117320.9179617
GGGCATT250.003416117320.9179616
GCGATTT456.3965854E-720.9179617
AACTGTC250.003416117320.9179619
ATGATTT250.003416117320.9179617
GTACTTT250.003416117320.9179617
Produced by FastQC (version 0.11.2)