FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_73.3510000005c628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_73.3510000005c628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18750
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC340.18133333333333332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCG17200.021.3423923
GGGTTCG556.330083E-921.342393
CTGGGTT556.330083E-921.342391
TGGGTTC556.330083E-921.342392
CTTGGTT16900.021.279251
TTGGTTC16950.021.2164782
TCGTTGG7400.020.9440027
GTTGATG250.003309842420.9449
GTGTTTT354.390078E-520.94418
GGGTTTT1000.020.94418
GTTGAGC250.003309842420.9449
GTTGACC250.003309842420.9449
GTTGACA250.003309842420.9449
CAGTTTT506.807386E-820.94418
TTCGTTG18550.020.9446
AATTTTT1300.020.94419
TTGTAGG250.003309842420.94410
TTATTTT250.003309842420.94418
TGATTTT354.390078E-520.94418
CGCTTTT250.003309842420.94418
Produced by FastQC (version 0.11.2)