FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_72.3510000005c60e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_72.3510000005c60e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33360
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGAGGCTGTCTCTTATAC2740.8213429256594724No Hit
GTGTAGTGTGGCTGTCTCTTATACACA2460.7374100719424461No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC1050.3147482014388489No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC1020.3057553956834532No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC890.2667865707434053No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC850.2547961630695444No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC720.2158273381294964No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC710.21282973621103118No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA650.19484412470023982No Hit
GTGTAGTGTGGACCTGTCTCTTATACA570.17086330935251798No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT480.14388489208633093No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC410.12290167865707433No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC370.11091127098321342No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC340.10191846522781775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTG404.3982436E-621.3859122
GTCGTGT1850.021.3859123
GTGTAGT29200.021.3859121
TGTAGTG29200.021.3859122
TGTCGTG1850.021.3859122
GTAGTGT30200.021.3150963
GGATGTG303.9026697E-420.93703310
GGAGAGG701.4551915E-1120.93703310
GGATGGG405.2914584E-620.93703310
GGATGGA303.9026697E-420.93703310
CAGTTTT354.5368364E-520.93703318
GGCATTT354.5368364E-520.93703317
ATCTTTT405.2914584E-620.93703318
TAGTGTG31000.020.9370334
AAATTTT354.5368364E-520.93703318
AATTTTT1350.020.93703319
GAGGAAG303.9026697E-420.93703311
CGGGGTT303.9026697E-420.93703316
GGCGTTT303.9026697E-420.93703317
GTTCTGT601.0004442E-920.93703320
Produced by FastQC (version 0.11.2)