FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_71.3510000005c5e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_71.3510000005c5e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62768
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA2730.4349349987254652No Hit
ATCTGCTATCCTGTCTCTTATACACAT780.12426714249299006No Hit
ATCTGCTATCCGGGGGGGGGTTTTTTT640.10196278358399186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCTA850.021.4408172
ATCTACT701.0913936E-1121.4408171
TCTACTA701.0913936E-1121.4408172
ACCTGCT353.8442282E-521.4408171
TCTGCTA57150.021.4408172
ATGCTAT850.021.4408173
ATATGCT850.021.4408171
ATTTGCT659.094947E-1121.4408171
ATCTGCT57150.021.4408171
TTTGCTA659.094947E-1121.4408172
CTACTAT701.0913936E-1121.4408173
ACTGCTA505.8384103E-821.4408152
ATCTCCT250.002964310621.4408151
CTCCTAT250.002964310621.4408153
AACTGCT505.8384103E-821.4408151
TCTCCTA250.002964310621.4408152
CTGCTAT58700.021.404293
TGGTTTT1800.020.92828818
GTATGTT303.9673573E-420.92828816
TGCATTT303.9673573E-420.92828817
Produced by FastQC (version 0.11.2)