FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_69.3510000005c5a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_69.3510000005c5a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53627
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC790.14731385309638803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGAAC1200.021.4416833
ATCTTGA800.021.4416831
ACATTGA556.684786E-921.4416831
ACTTTGA1200.021.4416831
TCTTGAA800.021.4416832
CTAGAAC658.913048E-1121.4416833
ACCTAGA658.913048E-1121.4416831
CTTGAAC49450.021.4416833
CTTTGAA1200.021.4416832
CCTAGAA658.913048E-1121.4416832
ACCTTGA48750.021.441681
CCTTGAA48800.021.4197142
AAATTTT558.789357E-920.94117718
AATTCTG354.596623E-520.94117719
TGATCTG405.382588E-620.94117718
TTATTTT405.382588E-620.94117718
TGATTTT701.4551915E-1120.94117718
GTATTTT850.020.94117718
CCGTTTT601.038643E-920.94117718
CGGATTT303.9404407E-420.94117717
Produced by FastQC (version 0.11.2)