FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_68.3510000005c586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_68.3510000005c586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48956
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTCCTACCTGTCTCTTATACACAT3170.6475202222403791No Hit
AGTCTCCTACGTCTGTCTCTTATACAC2810.5739848026799576No Hit
AGTCTCCTACGCTGTCTCTTATACACA1190.2430754146580603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCC658.54925E-1121.5105061
GCCTCCT658.54925E-1121.5105062
CCTCCTA658.54925E-1121.5105063
GTCTCCT44100.021.5105062
AGTCTCC44100.021.5105061
ACTCCTA505.574657E-821.5105043
AGTATCC353.726571E-521.5105041
TATCCTA353.726571E-521.5105043
TTCTCCT303.2799426E-421.5105042
AGGCTCC900.021.5105041
TCACCTA454.861304E-721.5105043
TCTCCTA44300.021.5105043
GCTCCTA900.021.5105043
AGACTCC505.574657E-821.5105041
AGTCCCC303.2799426E-421.5105041
ATTCTCC303.2799426E-421.5105041
GACTCCT505.574657E-821.5105042
AGTCACC454.861304E-721.5105041
TCCCCTA303.2799426E-421.5105043
TACGTCA2150.020.9172658
Produced by FastQC (version 0.11.2)