FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_65.3510000005c529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_65.3510000005c529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17368
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTGCGTATGCTGTCTCTTATACAC1070.6160755412252419No Hit
AACGTGCGTATGGCTGTCTCTTATACA650.37425149700598803No Hit
AACGTGCGTATGGGCTGTCTCTTATAC600.34546292031321973No Hit
AACGTGCGTATCTGTCTCTTATACACA540.31091662828189776No Hit
AACGTGCGTATGCTGTCTCTTCTACAC500.2878857669276831No Hit
AACGTGCGTATGCCTGTCTCTTATACA370.2130354675264855No Hit
AACGTGCGTATGAGCTGTCTCTTATAC260.14970059880239522No Hit
AACGTGCGTATGGCCTGTCTCTTATAC250.14394288346384154No Hit
AACGTGCGTATGACCTGTCTCTTATAC230.1324274527867342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGC16000.021.5341241
CGTGCGT16200.021.5341243
ACGTGCG16050.021.5341242
TTGTTTT303.827853E-420.91354618
GTATGTG1050.020.9135468
ATGGGGA250.003327887320.91354610
GTATGGT1400.020.9135468
ATGGGAG250.003327887320.91354610
GTATGCG1350.020.9135468
GTATGCA609.1677066E-1020.9135468
GTATGAT950.020.9135468
GTATGAG1000.020.9135468
CAGTTTT250.003327887320.91354618
AGCTTTT354.4147793E-520.91354618
AATTTTT900.020.91354619
TTTCTGT455.9037484E-720.91354621
TATGCGT354.4147793E-520.9135469
TATGCGC303.827853E-420.9135469
TATGCGA303.827853E-420.9135469
TATGTGG354.4147793E-520.9135469
Produced by FastQC (version 0.11.2)