FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_63.3510000005c4e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_63.3510000005c4e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46719
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA2410.5158500824075858No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT1200.2568548128170552No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC660.14127014704938035No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT660.14127014704938035No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT600.1284274064085276No Hit
TGGCATAGCTGAGGGGGCTGTCTCTTA510.10916329544724845No Hit
TGGCATAGCTGAGGGCTGTCTCTTATA490.10488238190029751No Hit
TGGCATAGCTGAGGGCCTGTCTCTTAT480.10274192512682205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAG303.3566504E-421.4342122
GACATAG250.002958090721.4342122
ACATAGC250.002958090721.4342123
TGGCGTA303.3566504E-421.4342121
GCGTAGC303.3566504E-421.4342123
GCATAGC40800.021.4342123
TTGCATA404.3760047E-621.434211
TGGCATA40400.021.434211
TGCATAG404.3760047E-621.434212
GTCATAG751.8189894E-1221.434212
GGCATAG40400.021.434212
GCCTAGC3200.021.434213
GGCCTAG3200.021.434212
TCATAGC751.8189894E-1221.434213
TGGCCTA3200.021.434211
GCTGAGG10150.020.9293388
TGAGACG354.6004057E-520.92933710
TGATGGT250.00339786920.92933710
TGATGGA303.9450353E-420.92933710
TGAGAAG354.6004057E-520.92933710
Produced by FastQC (version 0.11.2)