FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_60.3510000005c487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_60.3510000005c487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56189
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC2770.4929790528395237No Hit
GAATCCTTACGCTGTCTCTTATACACA700.12457954403886883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTTA800.021.2932153
GATTCCT800.021.2932151
ATTCCTT800.021.2932152
ATCCTTA51200.021.2932153
AATCCTT49950.021.2932132
CTCCTTA1550.021.2932133
ACTCCTT1550.021.2932132
TATCCTT1250.021.2932132
GAATCCT49950.021.2932131
AATACTT1400.021.2932132
GAATACT1400.021.2932131
ATACTTA1400.021.2932133
GACTCCT1550.021.2932131
GTAGATT250.003385134320.95191616
CGCGACT250.003385134320.95191610
CGCGACA250.003385134320.95191610
CGCTGGC701.4551915E-1120.95191610
GCGGGGA250.003385134320.95191611
TCCTTTT405.3664317E-620.95191618
GAGCGTT250.003385134320.95191616
Produced by FastQC (version 0.11.2)