FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_6.3510000005bdd1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_6.3510000005bdd1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75601
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA5960.788349360458195No Hit
CTGATCGTAGCACCTGTCTCTTATACA1690.22354201663999151No Hit
CTGATCGTAGCAGCTGTCTCTTATACA1030.13624158410603035No Hit
CTGATCGTAGCAGGGGCTGTCTCTTAT780.10317323844922686No Hit
CTGATCGTAGCAGGGGGGGGTTTTTTT760.10052777079668257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACG354.2174797E-521.1951891
CTTATCG1200.021.1951891
CTGACCG506.6445864E-821.1951871
CTGATCG67400.021.1951871
CTGCTCG3400.021.1951871
CCGATCG455.693E-721.1951871
TGACCGT506.739356E-821.1668912
TATCGTA1200.021.1668913
GAACGTA354.2612384E-521.1668913
TTATCGT1200.021.1668912
GACCGTA506.739356E-821.1668913
TGAACGT354.2612384E-521.1668912
TGATCGT67550.021.166892
GCTCGTA3400.021.166893
TGCTCGT3400.021.166892
GATCGTA69250.021.1668873
CGATCGT455.766833E-721.1668872
GGCGTTT1350.020.97090117
ATGTTTT1050.020.97090118
AGCATGC1050.020.9709019
Produced by FastQC (version 0.11.2)