FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_58.3510000005c444.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_58.3510000005c444.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44611
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC2430.544708704131268No Hit
ACGTCTCTGCCTGTCTCTTATACACAT1090.24433435699715314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCTC557.2595867E-921.2684881
ACGTATC3100.021.2684861
CGTATCT3100.021.2443182
ACGTCTC39650.021.2416671
GTCTCTG40250.021.2179263
CGTCTCT39700.021.2175622
CTCTGCA40950.020.958525
TTCATTT250.003368175820.9585217
ATGGGGG558.607458E-920.9585211
TGCATTA2250.020.958528
CATGATG250.003368175820.9585210
GTGTTTT1450.020.9585218
TGCATGA3050.020.958528
CAGTTTT354.5453467E-520.9585218
TGCATCC1850.020.958528
GGCATTT250.003368175820.9585217
TGCATAA2000.020.958528
CATTGGT250.003368175820.9585210
CATTGGG354.5453467E-520.9585210
CATTGGA354.5453467E-520.9585210
Produced by FastQC (version 0.11.2)