FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_57.3510000005c42a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_57.3510000005c42a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42505
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGTACGTAGGCTGTCTCTTATACA2600.6116927420303494No Hit
TACGGTACGTAGCCTGTCTCTTATACA1170.2752617339136572No Hit
TACGGTACGTAGTCTGTCTCTTATACA860.20232913774850014No Hit
TACGGTACGTAGCTGTCTCTTATACAC850.19997647335607574No Hit
TACGGTACGTAGACTGTCTCTTATACA690.16233384307728504No Hit
TACGGTACGTAGGGCTGTCTCTTATAC520.12233854840606986No Hit
TACGGTACGTAGGGGCTGTCTCTTATA490.1152805552287966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG709.094947E-1221.5006073
TACGATA709.094947E-1221.5006071
ACGATAC709.094947E-1221.5006072
ACGCTAC303.2800276E-421.5006052
TGGTACG404.2442844E-621.5006053
TACGGTA39100.021.5006051
ATGGTAC404.2442844E-621.5006052
CGCTACG303.2800276E-421.5006053
TACGCTA303.2800276E-421.5006051
ACGGTAC39100.021.5006052
CGGTACG39650.021.5006053
TATGGTA404.2442844E-621.5006051
TCGGTAC556.377377E-921.5006032
TTCGGTA556.377377E-921.5006031
GTAGAAG1050.020.9188259
AATTTTT2650.020.91882519
TACGTAG42300.020.9188256
GTAGATG507.439303E-820.9188239
GTAGATC405.389007E-620.9188239
GTAGATA558.767529E-920.9188239
Produced by FastQC (version 0.11.2)