FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_56.3510000005c41d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_56.3510000005c41d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47902
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC7221.5072439564110058No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC1660.34654085424408165No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC730.15239447204709614No Hit
ACAGTGCTCATGGCTGTCTCTTATACA720.1503068765396017No Hit
ACAGTGCTCCTGTCTCTTATACACATC640.13360611247964593No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC520.10855496638971233No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC510.10646737088221786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGC41850.021.4034161
ACTGTGC659.094947E-1121.4034161
CCGTGCT3100.021.4034162
ACAGAGC505.8718797E-821.4034161
ACCGTGC3100.021.4034161
CAGAGCT505.8718797E-821.4034162
AGTGCTC42100.021.4034163
CGTGCTC3100.021.4034163
CAGTGCT41850.021.4034162
TGTGCTC659.094947E-1121.4034163
ACAGCGC353.8711456E-521.4034141
AGCGCTC353.8711456E-521.4034143
CAGCGCT353.8711456E-521.4034142
ATGGGTA303.9408496E-420.9342410
TTGTTTT601.035005E-920.9342418
ATGGGCA303.9408496E-420.9342410
CATGACA303.9408496E-420.934249
CATGAAC303.9408496E-420.934249
AATTTTT1200.020.9342419
TGCGCGG303.9408496E-420.9342411
Produced by FastQC (version 0.11.2)