FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_55.3510000005c3e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_55.3510000005c3e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71784
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1690.2354285077454586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCA5150.021.2932032
TCCGCAA5150.021.2932033
CTTCCGC5150.021.2932031
CTACAGC455.436905E-721.2932011
ACAGCAA455.436905E-721.2932013
CTTCAGC59550.021.2932011
CGTCAGC404.6937785E-621.2932011
TACAGCA455.436905E-721.2932012
CCAGCAA800.021.2932013
CTCCAGC800.021.2932011
TCCAGCA800.021.2932012
ATCAGCA354.065925E-521.2932012
TCAGCAA60750.021.2932013
CTCAGCA354.065925E-521.2932012
TTCAGCA59650.021.2932012
GTCAGCA404.6937785E-621.2932012
TAAGCAA557.352355E-921.29323
TCGGCAA303.5362723E-421.29323
TTCTGCA303.5362723E-421.29322
TCTGCAA303.5362723E-421.29323
Produced by FastQC (version 0.11.2)