FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_54.3510000005c3cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_54.3510000005c3cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54875
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCGTAGCCTGTCTCTTATACACA4310.7854214123006834No Hit
CTGATCGTAGCACCTGTCTCTTATACA1020.18587699316628703No Hit
CTGATCGTAGCAGCTGTCTCTTATACA850.1548974943052392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT49350.021.1823222
CTGATCG49300.021.1823221
GCTCGTA2400.021.1823223
TGCTCGT2400.021.1823222
CAGATCG250.003175788621.1823221
CTGCTCG2400.021.1823221
AGATCGT250.003175788621.1823222
CCGATCG455.666752E-721.1823221
CGATCGT455.666752E-721.1823222
GAACGTA404.876465E-621.182323
GATCGTA50850.021.182323
CGGATCG800.021.182321
CTGAACG404.876465E-621.182321
TCCTTTT303.9057914E-420.9699218
GTGTCTG354.5508925E-520.9699218
GCCTTTT651.2187229E-1020.9699218
GGCATTT558.6638465E-920.9699217
ATCTTTT354.5508925E-520.9699218
TAAATTT250.00336733620.9699217
GGGCATT303.9057914E-420.9699216
Produced by FastQC (version 0.11.2)