FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_53.3510000005c3a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_53.3510000005c3a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29215
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC1380.47236008899537907No Hit
GAGCTACGTCTAGGCTGTCTCTTATAC530.18141365736779053No Hit
GAGCTACGTCTCTGTCTCTTATACACA450.154030463802841No Hit
GAGCTACGTCTAGGGCTGTCTCTTATA410.14033886702036624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTAC26450.021.4745181
GCTACGT27150.021.4745183
GAGATAC454.7968206E-721.4745181
ATCTACG404.2114716E-621.4745162
AGCTACG26500.021.4745162
GATCTAC404.2114716E-621.4745161
TGCTACG607.1486284E-1021.4745162
GGGTTTT1050.020.92294718
CAGTTTT303.9042506E-420.92294718
GGCATTT303.9042506E-420.92294717
CTATATT250.00337372120.92294710
ATCTTTT250.00337372120.92294718
TCTAGTT651.1641532E-1020.9229479
TCTAGGC609.931682E-1020.9229479
TCTAGGA250.00337372120.9229479
TCTAGCG507.224298E-820.9229479
TCTAGCA303.9042506E-420.9229479
TCTAGAT651.1641532E-1020.9229479
TCTAGAG507.224298E-820.9229479
ACGTCTC1000.020.9229476
Produced by FastQC (version 0.11.2)