FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_51.3510000005c37b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_51.3510000005c37b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37638
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC5221.3868962219033953No Hit
GAGGAACAGAGCTGTCTCTTATACACA1810.4808969658324034No Hit
GAGGAACAGAGTCTGTCTCTTCTACAC1560.4144747329826239No Hit
GAGGAACAGAGTGGCTGTCTCTTATAC420.11158935118762953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCAC3250.021.366351
GGCACAG3250.021.366353
GAGGAAC32150.021.366351
ATGAACA404.459138E-621.366352
AGGAACA32150.021.366352
GATGAAC404.459138E-621.366351
AGGCACA3250.021.366352
TGGAACA751.8189894E-1221.366352
TGAACAG404.459138E-621.366353
GGAACAG32950.021.366353
GTGGAAC751.8189894E-1221.366351
GTGTTTT701.4551915E-1120.9401618
GCCTTTT405.3144904E-620.9401618
CAGTTTT751.8189894E-1220.9401618
GAGTGTG651.1823431E-1020.940169
GAGTGTA405.3144904E-620.940169
ACGTTTT354.5515302E-520.9401618
GAGTGCG1150.020.940169
CGGGGTT354.5515302E-520.9401616
GAGTGCA303.9115825E-420.940169
Produced by FastQC (version 0.11.2)