FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_48.3510000005c31e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_48.3510000005c31e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43886
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC5461.2441325251788724No Hit
TAAGCGTGGAACTGTCTCTTATACACA510.11621018092330129No Hit
TAAGCGTGGAACGGGGGGGGTTTTTTT440.10025976393382854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGT38300.021.3132271
TAAGAGT1450.021.3132271
TGCGTGG1000.021.3132253
AAGCGTG38200.021.3132252
AGCGTGG38750.021.3132253
CGCGTGG1600.021.3132253
TAGCGTG557.075869E-921.3132252
AACGCTG303.9152073E-420.9486910
TGCATTT303.9152073E-420.9486917
ACTGTCG250.00337645220.9486911
AACGCGT250.00337645220.9486910
AACGTGG250.00337645220.9486910
AACGCCC250.00337645220.9486910
AACGCCA250.00337645220.9486910
AACGTCG250.00337645220.9486910
AACGTCA303.9152073E-420.9486910
AACGTAG303.9152073E-420.9486910
GCCTTTT507.341441E-820.9486918
GTCATTT250.00337645220.9486917
ATCTTTT303.9152073E-420.9486918
Produced by FastQC (version 0.11.2)