FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_47.3510000005c2f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_47.3510000005c2f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79813
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC1410.1766629496447947No Hit
TAGCAGACTGGACCTGTCTCTTATACA860.10775186999611593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGA557.044946E-921.380041
TTGCAGA659.458745E-1121.380041
AGAAGAC557.044946E-921.380042
TAGCTGA404.541418E-621.380041
GAAGACT557.044946E-921.380043
TAGCAGA69600.021.380041
ATCAGAC1300.021.380042
AGCAGAC69600.021.380042
GCAGACT70200.021.380043
TATCAGA1300.021.380041
TGCAGAC659.458745E-1121.380042
AGCTGAC404.541418E-621.380042
TAGCCGA4850.021.3800371
AGCCGAC4850.021.3800372
GCCGACT4900.021.1618753
GGATGTG405.444861E-620.93797110
GGATGTA250.003410659520.93797110
GATGGCG250.003410659520.93797111
GAGGTGG507.575727E-820.93797111
GTTTGTT250.003410659520.93797116
Produced by FastQC (version 0.11.2)