FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_45.3510000005c2bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_45.3510000005c2bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46454
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG41350.021.2554593
ATCTCGA40300.021.2554591
AGCTCGA404.706186E-621.2554591
ATCTTGA557.3323463E-921.2554591
CCTCGAC455.44318E-721.2554592
TATCGAC850.021.2554592
TTCGACG455.44318E-721.2554593
TCTCGAC40350.021.2554592
TCTTGAC557.3323463E-921.2554592
ACCTCGA455.44318E-721.2554591
ATATCGA850.021.2554591
ATTTCGA455.44318E-721.2554591
ATCGACG850.021.2554593
TTTCGAC455.44318E-721.2554592
CGTTTCT250.003370726720.95802119
TTGTTTT1000.020.95802118
GGTGATT250.003370726720.95802116
GAGGTCT250.003370726720.95802117
TGCATTT250.003370726720.95802117
GGCATTT250.003370726720.95802117
Produced by FastQC (version 0.11.2)