FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_44.3510000005c296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_44.3510000005c296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71516
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCAC506.4768756E-821.2423822
TTCGCCA2650.021.2423821
TAGCCAC62900.021.2423822
TCAGCCA506.4768756E-821.2423821
TCGCCAC2650.021.2423822
TTAGCCA62900.021.2423821
AGCCACT64900.021.242383
GAGCCAC1450.021.242382
TGCCACT900.021.242383
TTTGCCA900.021.242381
TTGCCAC900.021.242382
CTCTGAA2700.020.9601428
CTGGGTG1350.020.96014210
TCTGGTC1350.020.9601429
TCTGCGG2100.020.9601429
AGGGGTT250.003386390220.9601416
ACTGTTT250.003386390220.9601417
CTCTGGG13300.020.960148
CTCTGGC5200.020.960148
CTCTGGA4950.020.960148
Produced by FastQC (version 0.11.2)