FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_40.3510000005c21f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_40.3510000005c21f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45266
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC2650.5854283568241063No Hit
GTTGAGCTTGACCCTGTCTCTTATACA550.12150399858613528No Hit
GTTGAGCTTGACGCTGTCTCTTATACA520.11487650775416428No Hit
GTTGAGCTTGACGGGGGGGGTTTTTTT490.10824901692219327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGC404.4952885E-621.3652761
GTTGAGC37400.021.3652761
GTCGAGC353.918657E-521.3652761
AGAGCTT404.4952885E-621.3652763
TAGAGCT404.4952885E-621.3652762
TTGCGCT3000.021.3652762
TTGTGCT353.918657E-521.3652762
TCGAGCT353.918657E-521.3652762
GTTGCGC3000.021.3652761
GTTGTGC353.918657E-521.3652761
TTGAGCT37350.021.3652742
GTGGAGC1150.021.3652741
TGAGCTT38550.021.3375643
TGGTTTT1300.020.94033218
GCCTTTT601.027729E-920.94033218
CAGTTTT456.2806066E-720.94033218
AGCTTGA41600.020.9403325
GAAATTT303.9284574E-420.94033217
AAATTTT456.2806066E-720.94033218
TGCGTTT354.5777662E-520.94033217
Produced by FastQC (version 0.11.2)