FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_38.3510000005c1da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_38.3510000005c1da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71392
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA1750.2451255042581802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCGA1750.021.5191083
TGATGCC800.021.5191061
TTTTGCC658.731149E-1121.5191061
TGTTGCC63000.021.5191061
TTACCGA1450.021.5191063
GTTCCCG454.909343E-721.5191062
GATGCCG800.021.5191062
TGTTCCC454.909343E-721.5191061
TGCTGCC1100.021.5191061
TGTTACC1450.021.5191061
TTCCCGA454.909343E-721.5191063
ATGCCGA800.021.5191063
CTGCCGA1100.021.5191063
GTTACCG1450.021.5191062
TTTGCCG658.731149E-1121.5191062
GTTGCCG62950.021.5191062
TTGCCGA63550.021.5191063
TGGTGCC1800.020.9213541
GGTGCCG1800.020.9213542
ACAGTGC354.6675443E-520.91590910
Produced by FastQC (version 0.11.2)