FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_37.3510000005c1b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_37.3510000005c1b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64745
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGTACCGCTGTCTCTTATACACA2650.40929801529075605No Hit
CTCTAGTACCGAGGCTGTCTCTTATAC770.11892810255618196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGGT303.5294326E-421.294581
CTATAGT303.5294326E-421.294581
CTTTAGT751.8189894E-1221.294581
GCTAGTA950.021.294582
TCTGGTA303.5294326E-421.294582
CTCTAGT56850.021.294581
CTGGTAC303.5294326E-421.294583
ATAGTAC303.5294326E-421.294583
TTTAGTA751.8189894E-1221.294582
CTAGTAC58800.021.294583
TTAGTAC751.8189894E-1221.294583
CTCTTGT250.00308568621.2945791
TCTCGTA3150.021.2945792
TCTTGTA250.00308568621.2945792
CTTGTAC250.00308568621.2945793
CACTAGT506.2864274E-821.2945791
ACTAGTA506.2864274E-821.2945792
CCTAGTA506.2864274E-821.2945792
CTCGTAC3150.021.2945793
TCTAGTA56900.021.275872
Produced by FastQC (version 0.11.2)