FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_35.3510000005c160.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_35.3510000005c160.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98229
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA8120.8266397906931763No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA1260.12827169165928595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA5300.021.1748091
CAGAAGG88900.021.1748093
CACAGAA87450.021.1748091
ACAAAAG651.09139364E-1021.1748072
CACACAA404.9578775E-621.1748071
ACAGAAG87400.021.1748072
CACAAGG404.9578775E-621.1748073
CACAAAA651.09139364E-1021.1748071
ACACAAG404.9578775E-621.1748072
ATAGAAG1150.021.1748072
CAAAGAA506.7650035E-821.1748071
CATAGAA1150.021.1748071
CAAAAGG651.09139364E-1021.1748073
TTTTTTC405.3704916E-620.98064221
CCGTCTC250.003376107420.98064221
GTTTTCT303.9227426E-420.98064220
ATTTCTG507.458584E-820.98064220
ATTTTTT15050.020.98064220
ATTTTCT250.003376107420.98064220
TTTTCTG701.4551915E-1120.9806421
Produced by FastQC (version 0.11.2)