FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_34.3510000005c147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_34.3510000005c147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65272
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC2920.44735874494423333No Hit
ACTCGTCTCGATGCTGTCTCTTATACA680.10417943375413653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCATC751.8189894E-1221.3229351
TCATCTC751.8189894E-1221.3229353
CTCATCT751.8189894E-1221.3229352
TCCTCTC506.200389E-821.3229333
ACTCGTC58150.021.3229331
ACGTCTC608.385541E-1021.3229333
CTCCTCT506.200389E-821.3229332
ACTCCTC506.200389E-821.3229331
ACCCGTC1050.021.3229331
TAGTCTC1050.021.3229333
TCGTCTC58750.021.3229333
GCGTCTC1200.021.3229333
CTAGTCT1050.021.3229332
ACTAGTC1050.021.3229331
ACGCGTC1200.021.3229331
CTCGTCT58150.021.3229332
CGCGTCT1200.021.3229332
GTGTTTT2350.020.94712818
TCGATTC2350.020.9471288
TCGATGG9650.020.9471288
Produced by FastQC (version 0.11.2)