FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_33.3510000005c12d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_33.3510000005c12d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42112
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA1680.39893617021276595No Hit
AACGGAATGGCTGCTGTCTCTTATACA1000.23746200607902734No Hit
AACGGAATGGCTCCTGTCTCTTATACA930.22083966565349544No Hit
AACGGAATGGCTGTCTCTTATACACAT750.1780965045592705No Hit
AACGGAATGGCTCTGTCTCTTATACAC630.14960106382978725No Hit
AACGGAATGGCTACTGTCTCTTATACA580.13772796352583586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAA607.694325E-1021.4155541
AGGAATG455.0381277E-721.4155543
AAAGGAA455.0381277E-721.4155541
CGAAATG607.694325E-1021.4155543
AAGGAAT455.0381277E-721.4155542
ACGAAAT607.694325E-1021.4155542
AACGCAA353.8395337E-521.4155521
ACGCAAT353.8395337E-521.4155522
CGGAATG39050.021.4155523
ATCGGAA505.7931175E-821.4155521
AATGGAA505.7931175E-821.4155521
AACGGAA38500.021.4155521
CGCAATG353.8395337E-521.4155523
ACGGAAT38550.021.4155522
CTCGACG303.932332E-420.93230411
TGGTTTT850.020.93230418
CCAATTT303.932332E-420.93230417
GTGTTTT850.020.93230418
GGGTTTT1550.020.93230418
CAATGGC354.581516E-520.9323045
Produced by FastQC (version 0.11.2)