FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate2_30.3510000005c0db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate2_30.3510000005c0db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63364
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC2470.38981124928981753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGACG800.021.5716613
TGTGACG56250.021.5716613
GTATGAC353.6681784E-521.5716612
GGTGACG1600.021.5716613
GAGTGAC1000.021.5716612
TATGACG353.6681784E-521.5716613
AGTATGA353.6681784E-521.5716611
AGAGTGA1000.021.5716611
ATTGTGA353.6681784E-521.5716611
AGTGTGA55750.021.5716611
AGTGAGA800.021.5716611
TTGTGAC353.6681784E-521.5716612
GTGTGAC55800.021.5716612
GGGTGAC1600.021.5716612
GCGTGAC1150.021.571662
AGCGTGA1150.021.571661
CGTGACG1150.021.571663
GAACGCG2150.020.9076589
CCGTTTT1050.020.90765818
CGAACTA2150.020.9076588
Produced by FastQC (version 0.11.2)